Recurrent Isolation of Extremotolerant Bacteria from the Clean Room Where Phoenix Spacecraft Components Were Assembled
نویسندگان
چکیده
منابع مشابه
Recurrent isolation of extremotolerant bacteria from the clean room where Phoenix spacecraft components were assembled.
The microbial burden of the Phoenix spacecraft assembly environment was assessed in a systematic manner via several cultivation-based techniques and a suite of NASA-certified, cultivation-independent biomolecule-based detection assays. Extremotolerant bacteria that could potentially survive conditions experienced en route to Mars or on the planet's surface were isolated with a series of cultiva...
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Spore-forming microorganisms are of concern for forward contamination because they can survive harsh interplanetary travel. Here, we report the draft genome sequences of 12 spore-forming strains isolated from the Manned Spacecraft Operations Building (MSOB) and the Vehicle Assembly Building (VAB) in Cape Canaveral, FL, where the Viking spacecraft were assembled.
متن کاملIsolation and characterization of bacteria capable of tolerating the extreme conditions of clean room environments.
In assessing the bacterial populations present in spacecraft assembly, spacecraft test, and launch preparation facilities, extremophilic bacteria (requiring severe conditions for growth) and extremotolerant bacteria (tolerant to extreme conditions) were isolated. Several cultivation approaches were employed to select for and identify bacteria that not only survive the nutrient-limiting conditio...
متن کاملDescription of Tersicoccus phoenicis gen. nov., sp. nov. isolated from spacecraft assembly clean room environments.
Two strains of aerobic, non-motile, Gram-reaction-positive cocci were independently isolated from geographically distinct spacecraft assembly clean room facilities (Kennedy Space Center, Florida, USA and Centre Spatial Guyanais, Kourou, French Guiana). A polyphasic study was carried out to delineate the taxonomic identity of these two isolates (1P05MA(T) and KO_PS43). The 16S rRNA gene sequence...
متن کاملCultivation of anaerobic and facultatively anaerobic bacteria from spacecraft-associated clean rooms.
In the course of this biodiversity study, the cultivable microbial community of European spacecraft-associated clean rooms and the Herschel Space Observatory located therein were analyzed during routine assembly operations. Here, we focused on microorganisms capable of growing without oxygen. Anaerobes play a significant role in planetary protection considerations since extraterrestrial environ...
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ژورنال
عنوان ژورنال: Astrobiology
سال: 2010
ISSN: 1531-1074,1557-8070
DOI: 10.1089/ast.2009.0396